Multiple reaction monitoring (MRM), also known as selected reaction monitoring (SRM), and parallel reaction monitoring (PRM) are targeted proteomics technologies aimed at quantifying up to hundreds of proteins in the same experiment. In contrast with hyper reaction monitoring (HRM™), these techniques are not discovery‑base, and are meant to quantify a panel of selected proteins.
Multiple reaction monitoring (MRM)
In the MRM workflow, proteins are analyzed on a triple quadrupole mass spectrometer. The quadrupole 1 (Q1) is used as a mass filter to select a given precursor which is fragmented in quadrupole 2 (Q2). In quadrupole 3 (Q3), a predefined number of product ions from every precursor are analyzed. This enables highly sensitive protein quantification over seven orders of magnitude.
Parallel reaction monitoring (PRM)
PRM differs from MRM on the fact that all fragment ions instead of only selected ones are measured after fragmentation of a selected precursor. For this reason, PRM is typically performed on Orbitrap or Time of flight (ToF) analyser.
Are MRM and PRM suitable to do absolute quantification?
Both technologies are suitable to perform absolute quantification by using stable isotope heavy labeled reference peptides spiked into the sample at known concentrations.
SpectroDive™ is Biognosys' software solution to analyze MRM and PRM data. SpectroDive will assist you from library and panel generation, MS method setup, to data analysis and results interpretation. To know more about it, you can keep on reading here.
Created by SEZ. Last update 2018-03-15 by SEZ