Biognosys hyper reaction monitoring (HRM™) technology refers to the combination of a comprehensive data independent acquisition (DIA) on a high resolution platform, the use of iRT spectral libraries, and data extraction using Spectronaut™ Pulsar.
The advantages of HRM over shotgun proteomics (data dependent acquisition or DDA) are many. You may want to start reading this article here to know the main differences between DIA and DDA. Biognosys’ DIA workflow (HRM) considerably outperforms DDA in most cases. HRM is more sensitive, meaning more peptides are identified in a single run. The dynamic range of peptide precursor detection in a typical single shot. HRM LC-MS run spans four to five orders of magnitude, with a LLOQ in the range of 10s of attomole on column. In addition, it produces data of higher quality in terms of variability, reproducibility, and completeness. HRM produces data with median CVs of 5‑10% for peptides and of 5% for proteins.
To know more about our technology, please visit our ScienceHub here.
Created by SEZ. Last update 2018-03-15 by SEZ