If you do or are planning to do mass spectrometry based proteomics, we are confident our solutions will help you getting the best out of it. Whether you are planning a discovery based proteomics project or a targeted approach to quantify a specific set of proteins, we offer superior solutions from the sample preparation to the data analysis and interpretation.
Sample Preparation Kit
The Sample Preparation Kits provide a reliable method for high throughput preparation of samples for any mass spectrometry proteomics workflow: multiple reaction monitoring (MRM), parallel reaction monitoring (PRM), hyper reaction monitoring (HRM™), data dependent acquisition (DDA) or shotgun approach, etc. Our kits will help minimizing variability and optimizing reproducibility. The protocols include all steps in the sample preparation workflow to deliver ready to inject samples. The Sample Preparation Kit Pro is optimized for mammalian cell culture, plasma and serum sample preparation. To find more information about or Sample Preparation Kits, click here.
Spectronaut™ Pulsar and directDIA™
If you’re interested in a discovery-based proteomics approach, DIA workflows have been shown to provide the most comprehensive proteome coverage (Bruderer et al. 2015). To know more about DIA and how it surpasses traditional DDA (shotgun) for discovery based approaches, click here.
Biognosys has made DIA data easy to analyze with the development of Spectronaut Pulsar -the most powerful and intuitive software solution for DIA approaches. Spectronaut Pulsar allows a spectral library‑free workflow, directDIA, enabled by our integrated database search engine, Pulsar. Alternatively, Spectronaut Pulsar also allows you to run a spectral library‑based targeted extraction of your DIA data (HRM). If you want to know more about Spectronaut Pulsar, keep on reading about it here or visit our site here. If you need help in deciding which workflow suits you best, do not hesitate to contact us here.
If you already know which proteins or peptides you want to quantify, a targeted approach is probably what suits you best, either MRM or PRM. Our targeted proteomics software solution SpectroDive, will assist you from the library and panel generation through the MS method set‑up to signal processing and interpretation of your data. SpectroDive features the smartest peak picking algorithm to minimize manual data curation. Further, it contains statistical data analysis and a complete quality control monitoring interface. To get more information about targeted workflows and SpectroDive, visit our site here or read more about it here.
To keep you focused on the science, we have developed QuiC, a simple tool that generates easy to interpret quality control readouts in near real time. QuiC can regularly check folders for new raw files which are used to continuously monitor instrument performance. A multitude of readouts are provided, the most important ones based on the iRT peptides. This tool allows you to easily and efficiently detect pitfalls in the workflow to help you prevent sample loss, LC‑MS downtime, and poor data quality data. QuiC is free of charge and only needs the iRT peptides to run. Visit our webshop here to request your QuiC license!
All Biognosys’ workflows benefit remarkably from the use of the iRT Standards contained in the iRT Kit. iRT ‑for indexed Retention Time‑ is a dimensionless value that defines, for a given resin type (e.g. C18), the chromatographic retention time of a peptide relative to the standards. In contrast to empirical retention time, the iRT value of a peptide is stable and enables accurate prediction of peptide retention for any chromatographic setup. This is possible thanks to the iRT peptides: eleven non‑naturally occurring synthetic peptides which have been optimized for stability, sensitivity, and even retention time spacing over the gradient.
By calibrating your LC system with the iRT Kit, you will dramatically increase the throughput of your targeted proteomics (MRM and PRM) experiments ‑up to 20 times‑ by bundling more transitions into one run. In HRM workflows, iRT peptides allow high‑quality spectral library generations and significantly more precise and efficient data analysis by using them as anchor points for data extraction.
These are, however, not the only applications for iRT peptides. Due to their highly stable behavior in LC‑MS setups, they are further used for QC monitoring purposes. The easiest and most effective way of profiting from this feature is the use of QuiC.
At Biognosys, we have developed ready to use assay panels for the screening of common cancer, cardiovascular, or metabolic biomarkers. PlasmaDeepDive multiplexed assay enables absolute quantification of 100 human plasma proteins in a single measurement in scheduled MRM or PRM experiments, covering a dynamic range of six orders of magnitude, with high precision and reproducibility. It includes 16 peptides corresponding to the typically depleted proteins in human plasma analysis, which can be used to monitor the depletion reproducibility and quality, nine housekeeping proteins for normalization, making the results highly reproducible, and 75 proteins that represent relevant targets for biomarker screening.
The PlasmaDeepDive multiplexed assay also provides all the reagents, material, and protocol to assist you from a reproducible sample preparation to absolute quantification of your targets and interpretation of your results. Additional information on the targeted proteins and use cases can be found in the ScienceHub section on our website here.
Are you unfamiliar with proteomics or do you lack the time or resources to carry out your proteomics project? We provide contract research services for high‑content protein quantification from any species, in any biological matrix. Our team is ready to develop a research plan with you that’s tailored to your research objectives. Our projects are carried out in our state of the art proteomics facility. Whatever your needs are, either the analysis of entire proteomes or quantification of specific proteins, we have the resources and the expertise to find the right solution together.Learn more about our contract research services: visit our site here or contact us here.
For more Biognosys solutions, visit our shop here.
Bruderer R, Bernhardt OM, Gandhi T, Miladinović SM, Cheng L-Y, Messner S, Ehrenberger T, Zanotelli V, Butscheid Y, Escher C, Vitek O, Rinner O, and Reiter L (2015) Extending the Limits of Quantitative Proteome Profiling with Data-Independent Acquisition and Application to Acetaminophen-Treated Three-Dimensional Liver Microtissues. Mol Cell Proteomics 14:1400–1410.
Created by SEZ. Last update 2018-03-15 by SEZ